9th WORLD CONGRESS ON ALTERNATIVES AND ANIMAL USE IN THE LIFE SCIENCES

cellasys will present two posters at the “9th World Congress on Alternatives and Animal Use in the Life Sciences“:

1. Serial Linkage of BioChips toward an organ-on-chip approach

2. Using chlorella kessleri for monitoring of water quality


Serial Linkage of BioChips toward an organ-on-chip approach
The development of drugs is a costly and time consuming process. Although high throughput methods based on cell cultures are available, they only give a hint on the real effect of a future drug. Traditional two dimensional cell cultures are not able to reproduce/emulate the complex interactions of different types of tissue like it exists in the human body. Such interactions include the absorption, distribution, metabolism and elimination (ADME) of a drug. Recently new approaches reforming the cell culture techniques were published. Most of them are based on several microchambers that are cultivated with different cells. Microfluidic channels are used to link these chambers and to ensure exchange of metabolites. With such devices e.g. primary cells can be cultivated for a longer time period and with a smaller loss of functionality than in former single-type 2D cell-cultures. A lable-free long term monitoring of organ-on-chip systems would affirm the benefits of such systems. Electrochemical sensors are well suited for this task, but need to be careful integrated. As proof-of-concept we serially linked two electrochemical cell-monitoring systems (IMOLA-IVD, cellasys GmbH) and showed that the exchange of metabolites can be monitored without any crosstalk in a label-free, long-term and multi-parametric manner.

Using chlorella kessleri for monitoring of water quality
Polution of surface waters is an increasing problem in the modern world. Causes are various like over-fertilization, insufficient capabilities or lack of wastewater treatment plants, littering, ecological disaster or eaven chemical and biological warfare. In the presented work the algae chlorella kessleri was used as an unspecific signal transducer for water quality. The algae was immoilized on a BioChip with a cellulose membrane, supplied with algae culture broth and monitored using the IMOLA-IVD technology which was developed in cooperation with the Heinz Nixdorf Lehrstuhl für Medizinische Elektronik at Technische Universität München. Here, the oxygen production due to photosynthetic stimulation of the algae with light emitting diodes and the extracellular acidification is monitored. If the water quality changes, the photosynthetic activity of the algae is also altered and that can be detected with the set-up. In a proof of principle study, probes from Indonesian palm plants were investigated. The technology showed that it can be used as an unspecific early warning system for water quality monitoring. The technology and first results using probes from Indonesian palm plants are presented. Future work will be on the transition from the laboratory to a real world scenario.

Ω 8

New premises for cellasys – R&D


cellasys – R&D moved to new premises at

cellasys – R&D

Ohmstrasse 8
80802 Munich Germany

BIOCHIP-D

BioChip-D data sheet available.


The data sheet of our new BioChip-D is available. The BioChip is an improved version of the BioChip-C. The substrate is made of glass and allows access via optical techniques.

NEXT FACTORY WEBSITE

“All-in-one manufacturing platform for system in package and micromechatronic systems” – project website is availabe at www.nextfactory-project.eu


The NextFactory project (funded from the European Union’s Seventh Framework Programme) aims to develop one machine which is able to produce complete mechatronic micro-systems in an integrated, single-stage, one-location process.

More information in the NextFactory-Factsheet.

ANALYTICA 2014

Booth B2.311

9. KOLLOQUIM PROZESSANALYTIK IN LUDWIGSHAFEN.

cellasys will present two posters at the “9. Kolloquium Prozessanalytik in Ludwigshafen” from 28. – 29.11.2013 in Ludwigshafen / Germany


Please visit our posters

– BioChip-H: Multiparametric BioChip for Process Analysis

and

– 8-Kanal-Multipotentiostat für die Analyse von Mikroelektroden.

VISITING LECTURE AT UNIVERSITY OF RIAU

A visiting lecturer from 18.11.2013 till 22.11.2013 will take place at University of Riau, Pekanbaru – Indonesia including several courses on label free monitoring of living cells.


For details please contact info@cellasys.com.

18th EUROPEAN CONGRESS ON ALTERNATIVES TO ANIMAL TESTING

cellasys will present two posters at the “18th European Congress on Alternatives to Animal Testing”:

1. “Monitoring of multilayer development of human 3D cornea constructs by trans-epithelial impedance measurement” together with the Institut für angewandte Zellkultur, Dr. Toni Lindl GmbH

2. “Comparison of standard and fetal-calf-serum-free cell culture media by impedance measurement” together with the Deutscher Tierschutzbund – Akademie für Tierschutz


Monitoring of multilayer development of human 3D cornea constructs by trans-epithelial impedance measurement
Measurement of transepithelial electrical resistance (TEER) is a widely accepted method to monitor living epithelial cells in vitro. In the presented work we used a modified IMOLA-IVD system to monitor the multilayer development of human cornea epithelial (HCE) constructs at the air/liquid interface by means of transepithelial impedance (real and imaginary part). This is a useful method in the field of toxicology to study the effect of chemicals towards 3D- constructs of living cells. The method is highly sensitive, label-free, non invasive, reproducible and delivers additional information without the use of further histological analysis.

Comparison of standard and fetal-calf-serum-free cell culture media by impedance measurement
Although considerable progress is made in the development of synthetic media for culturing cells, fetal calf serum (FCS) is still routinely used as the standard supplement for cell cultures. FCS is extracted from the blood of fetal calves thus raising strong ethical concerns. An abundance of scientific literature shows that fetuses are capable of experiencing pain and distress. From the animal welfare point of view the use of FCS therefore is not acceptable and researchers should be obliged to exploit all possibilities to grow their cells in media free of FCS. Consequently, in particular the use of FCS in standard protocols should be critically examined and replaced wherever possible.

We investigated whether the culturing of the L929 permanent mouse fibroblast cell line is possible using commercially available synthetic media, not containing FCS. Standard protocols for this cell line such as in various INVITTOX protocols require addition of 10% FCS into the cell culture medium. We used bioimpedance as an indicator of cell viability.

BIOCHIP-H AND CONTACT-SET

Our new Biochip-H and a simple contact-set are available


BioChip-H:
Improved multiparametric BioChip (pH, pO2, Impedance, MEA, Stimulation-electrodes and temperature) for measurement of cellular vitality and changes in bioimpedance on glass substrate for optical access via microscope.

EMBC’13

Meet us at the “35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society“.


cellasys will contribute with an invited talk “Label-Free Monitoring of Whole Cell Vitality” and a poster entitled “Evaluation of an Inkjet Printed Biochip”.

Label-Free Monitoring of Whole Cell Vitality
The Intelligent Mobile Lab (IMOLA) delivers metabolic and morphological parameters of living cells in a label-free and real time way. It represents a key technology for the development of new cell-based assays. Electrochemical microsensors are used to measure the extracellular acidification (pH), cellular respiration (pO2), changes in cell number and morphology (electric impedance) in a controlled environment. These parameters are closely linked to the intracellular signaling network of the living cells. They are thus likely to respond sensitively to changes in cellular vitality. A wide spectrum of cell types can be tested with the system, including adherent and suspended cells, continuous cell lines, primary cells or tissue samples. The platform is described in detail and applications in the field’s oncology, toxicology and environmental monitoring are shown.

Evaluation of an Inkjet Printed Biochip
Micro-sensor biochips have various applications in the field of live sciences. In this work, we present first results from a biochip manufactured in inkjet technology. The micro-sensor, contacts and conductors of this biochip were printed with an inkjet process onto a flexible Kapton substrate. The micro-sensor is an interdigitated electrode structure comprising of a mixture of carbon nanoparticles and multi-walled carbon nanotubes. In our project the reaction of the structure toward changes in pH, dissolved oxygen concentration, glucose and lactate was evaluated.