Researchers from Indonesia published “Amperometric microbial biosensor for sugars and sweetener classification using principal component analysis in beverages” with cellasys’s BioChips.
Weber, T., Wiest, J., Oredsson, S., Bieback, K.: Case Studies Exemplifying the Transition to Animal Component-free Cell Culture, Alternatives to Laboratory Animals, 2022, doi:10.1177/02611929221117999
Please visit the poster “Reproducibility in Cell Culture: Replacing Fetal Bovine Serum” at the 21st International Congress of the European Society of Toxicology In Vitro (ESTIV 2022) in Barcelona-Sitges / Spain.
We will be present at EUSAAT 2022 with the talk “cellasys #8: A microphysiometric test to identify serum-free cell culture media” presented by Dr. Sebastian Eggert.
cellasys will participate with the following contributions to the Microphysiological Systems World Summit 2022:
Talk: Systems engineering of microphysiometry
Poster: The cellasys #8 assay: An automated and standardized assay to address recovery effects
Poster: Reproducibility in Cell Culture: Replacing Fetal Bovine Serum
We look forward to meet you in New Orleans.
The evaluation of new cell culture media is usually conducted via weaning experiments, which require several weeks to acquire results for new media formulations. To accelerate the identiﬁcation process and, hence, the successful development of new media formulations, we describe the cellasys #8 assay, which is a standardized microphysiometry method to evaluate enhanced cell culture media within 24 hours. As an example, the transition to a chemically deﬁned media without serum is shown using L929 cells.
Welcome to “Heart-on-a-Chip: Microphysiometric monitoring of cardiomyocytes differentiated from human induced pluripotent stem cells” at the 7th German Pharm-Tox Summit.
Eggert, S.1, Hariharan, K.2, Wiest, J.1
1 cellasys GmbH, Kronburg / Germany
2 Fraunhofer-Institute for Biomedical Engineering (IBMT) – Fraunhofer Project Center for Stem Cell Process Engineering, Würzburg / Germany
Microphysiometry is the measurement of the functions and activities of life or of living matter and of the physical and chemical phenomena involved. It allows label-free monitoring of cellular metabolism and morphology of living cells and enables new applications in fields such as pharmacology or toxicology. Recently, we were able to extend the method toward monitoring of 3D cellular models, referred to as Organ-on-a-Chip.
To demonstrate the applicability of microphysiometry with human cardiomyocytes, a cellasys #8 assay  was performed via label-free and non-destructive monitoring of metabolic and morphological changes. IBMT cardiomyocytes from human induced pluripotent stem cells (hiPSCs) were cultivated on BioChips from day 22 to day 30. After transportation of the BioChips from Würzburg to Munich and overnight incubation, the cardiomyocytes were successfully checked optically for their beating activity. Then, a cellasys #8 assay was performed to identify changes after the transition from a serum-based to a chemically defined media without serum.
During the 24h cellasys #8 assay, the extracellular acidification and changes in impedance of the cells were monitored in real-time. The FBS-free medium caused a decrease in the metabolic activity; however, the impedance values did not change essentially. The results indicate that the FBS-free test medium is a candidate for further use in more defined experiments using hiPSCs.
The proof-of-principle measurement with human induced pluripotent stem cells demonstrated that microphysiometry is a promising tool for use in pharmacology and toxicology. In addition, it was possible to show that hiPSCs keep their functionality after a half day transportation between laboratories.