The combination of classical engineering disciplines and knowledge from cell biology allows the construction of physiologically relevant models to predict processes in the human body in-vitro. In the symposium different aspects of tissue-on-a-chip systems are presented. These include a biopsy-based approach to develop personalized therapies for cancer patients. A bone-on-a-chip that allows the investigation of 3D bone organoids under mechanical load and it’s optimization using in-silico technologies. Further, the necessity of including immune cells into models of osteochondral tissues will be discussed. Examples about applications of dynamic magnetic fields to improve transfection and apply mechanical force inside living cells, a case study about a tissue-on-a-chip system for skin and intestine for applications in toxicology and aspects to employ living cells as biosensors in microphysiometrical systems are on the agenda.
At IEEE EMBC 2019 in Berlin / Germany cellasys co-organizes a symposia on:
Cells and tissue as prediction model for toxicology, drug development and personalized medicine ( organized by J. Wiest and F. Schulze )
M. Brischwein: Development of personalized therapies for cancer with 3D tumor spheroids
Multicellular tumor spheroids are 3D models generated from various cell types, including heterogeneous tissues of solid human tumors. They have proven utility for predictive testing of patient samples to identify the most promising pharmaceutical anti-cancer treatment.
F. Schulze: Mechanical loading of human osteoblasts in a bone-on-a-chip
Organ-on-chip systems are used to recreate the physiology of a certain tissue or organ of interest, thereby enabling more detailed investigations than conventional cell culture allows for. In this project a bone-on-a-chip system is presented that allows for control of oxygen levels and mechanical loading
A. Lang: Modelling the crosstalk between immune cells and bone
Interaction between immune cells and bone metabolism plays a crucial role in the pathogenesis of several disorders affecting the musculoskeletal system. However, current engineering approaches exclude the immune system due to its complexity. Here, we aim to present the importance of modelling the crosstalk between immune cells and bone as exemplified by mimicking the initial phase of fracture healing in vitro.
M. Koch: New magnetic field device for application with laser microscopes
A new digitally controlled field generator is integrated into a laser microscope. Magnetic particles powered non-invasively by the field generator treat cell populations inside a dish above the microscope lens, which can be observed in real time.
J. Wiest: Tissue-on-a-chip
Microphysiometry is a powerful tool to monitor the energy metabolism and changes in morphology of living cells. Until now, the technique was mainly used for monitoring of cells in 2D monolayers. Now, the cellasys group showed that it is possible to monitor the extracellular acidification rate (EAR) and transepithelial electrical resistance (TEER) of 3D skin constructs in an automated assay maintaining an air liquid interface (ALI) with the IMOLA-IVD technology.