18th EUROPEAN CONGRESS ON ALTERNATIVES TO ANIMAL TESTING

cellasys will present two posters at the “18th European Congress on Alternatives to Animal Testing”:

1. “Monitoring of multilayer development of human 3D cornea constructs by trans-epithelial impedance measurement” together with the Institut für angewandte Zellkultur, Dr. Toni Lindl GmbH

2. “Comparison of standard and fetal-calf-serum-free cell culture media by impedance measurement” together with the Deutscher Tierschutzbund – Akademie für Tierschutz


Monitoring of multilayer development of human 3D cornea constructs by trans-epithelial impedance measurement
Measurement of transepithelial electrical resistance (TEER) is a widely accepted method to monitor living epithelial cells in vitro. In the presented work we used a modified IMOLA-IVD system to monitor the multilayer development of human cornea epithelial (HCE) constructs at the air/liquid interface by means of transepithelial impedance (real and imaginary part). This is a useful method in the field of toxicology to study the effect of chemicals towards 3D- constructs of living cells. The method is highly sensitive, label-free, non invasive, reproducible and delivers additional information without the use of further histological analysis.

Comparison of standard and fetal-calf-serum-free cell culture media by impedance measurement
Although considerable progress is made in the development of synthetic media for culturing cells, fetal calf serum (FCS) is still routinely used as the standard supplement for cell cultures. FCS is extracted from the blood of fetal calves thus raising strong ethical concerns. An abundance of scientific literature shows that fetuses are capable of experiencing pain and distress. From the animal welfare point of view the use of FCS therefore is not acceptable and researchers should be obliged to exploit all possibilities to grow their cells in media free of FCS. Consequently, in particular the use of FCS in standard protocols should be critically examined and replaced wherever possible.

We investigated whether the culturing of the L929 permanent mouse fibroblast cell line is possible using commercially available synthetic media, not containing FCS. Standard protocols for this cell line such as in various INVITTOX protocols require addition of 10% FCS into the cell culture medium. We used bioimpedance as an indicator of cell viability.

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