Applications flyer

- Drug testing (e.g. chemosensitivity)

To test the efficiency of a drug previous to the start of a therapy the reaction of an individual biopsy toward the drug can be detected with the IMOLA system. The graph at the right side shows the effect of doxorubicin toward MCF-7 cells compared to a control experiment.

Wolf, B., Brischwein, M., Lob, V., Ressler,J., Wiest,J.: Cellular Signalling: Aspects for Tumor Diagnosis and Therapy. Biomedizinische Technik, Volume 52, Issue 1, 164-168, 2007

Brischwein, M., Geisler, T., Lob, V., Wiest, J., Ressler, J., Wolf, B.: Chip statt Maus: Microsensorarrays zur Chemikalienprüfung. Nachrichten aus der Chemie 54, 115-120, Feb 2006

Wiest, J., Brischwein, M., Ressler, J., Otto, A.M., Grothe, H., Wolf, B.: Cellular Assays with Multiparametric Bioelectronic Sensor Chips. Chimia 59, 243-246, 2005

Henning, T., Kraus, M., Brischwein,M., Otto, A.M., Wolf, B.: Relevance of Tumor Microenvironment for progression, therapy and drug development. Anti-Cancer Drugs 15, 7-14, 2004

Brischwein, M., Motrescu, E.R., Otto, A.M., Cabala, E., Grothe, H., Wolf, B.: Functional Cellular Assays with Multiparametric Silicon Sensor Chips. Lab on a Chip 3 (4), 234-240, 2003

- Environmental monitoring (e.g. water quality)

Using algae and the BioChip-C in an IMOLA-system one can detect the presence of toxins in water. Applications range from monitoring of drinking water quality toward the measurement at sewage plants to shelter its biology.

Stadthagen, T. Entwicklung eines online Gewässermonitoringsystems mittels Biosensorchips zum Nachweis ausgewählter Xenobiotika, Dissertation - Technische Universität München

Wiest, J., Stadthagen, T., Schmidhuber, M., Brischwein, M., Ressler, J., Raeder, U., Grothe, H., Melzer, A., Wolf, B. Intelligent Mobile Lab for Metabolics in Environmental Monitoring, Analytical Letters, Volume 39, Issue 8, Jul 2006, Pages 1759 - 1771, DOI 10.1080/00032710600714089, URL http://dx.doi.org/10.1080/00032710600714089

J. Wiest, M. Schmidhuber, D. Grundl, M. Brischwein, H. Grothe, B. Wolf: Environmental Engineering using Living Cells as Signal Transducers, IEEE Africon 2007, Windhoek/Namibia, ISBN: 0-7803-8606-X, IEEE Catalog number: 04CH37590C

- Toxicokinetic experiments (e.g. alternative method for animal experiments)

To avoid animal experiments it is possible to perform experiments in toxicology using living cells. The figure aside shows the change in extracellular acidification of 3T3 fibroblasts after addition of mercury. Furthermore it is possible to monitor the recovery phase of the cells after removal of the toxin.

M. Zottmann, B. Becker, J. Wiest, D. Grundl, M. Schmidhuber, A.M. Otto, B. Wolf: Sensor chip-based test systems for multiparametric real time monitoring of cell metabolism and drug response, European Journal of Cell Biology, 2009, 88, Suppl. 59, 23

J. Wiest, D. Grundl, M. Schmidhuber, M. Brückl, M. Brischwein, H. Grothe, A.M. Otto, B. Wolf: Cell based assay for label-free, long-term investigation of living cells as alternative testing method for toxicity, ALTEX, 2008, Suppl. Linz, 1, 77-78

Otto, A.M., Brischwein, M., Motrescu, E., Cabala, E., Grothe, H., Stepper, C., Wolf, B. (2004): Chips statt Mäuse: Zellen auf bioelektronischen Sensorchips als Alternative zu Tierversuchen. ALTEX , 21 (Suppl. Linz 3) 70-76.