Tierversuche zählen zu den umstrittensten Praktiken, in denen wir Tiere nutzen, insbesondere weil den Tieren hier absichtlich Schmerzen, Leiden und Schäden zugefügt werden – mit der Begründung eines möglichen Erkenntnisgewinns für den Menschen. An dieser Rechtfertigung für die Unerlässlichkeit von Tierversuchen hat sich wenig geändert, und das, obwohl nicht nur die ethischen Probleme, sondern auch die wissenschaftlichen Mängel von Tierversuchen mittlerweile offensichtlich und gut dokumentiert sind. Dieses Wissen über die Probleme im Zusammenhang mit Tierversuchen ist allerdings noch nicht weit verbreitet, und es besteht die Gefahr, dass es schlicht ignoriert wird.
The combination of classical engineering disciplines and knowledge from cell biology allows the construction of physiologically relevant models to predict processes in the human body in-vitro. In the symposium different aspects of tissue-on-a-chip systems are presented. These include a biopsy-based approach to develop personalized therapies for cancer patients. A bone-on-a-chip that allows the investigation of 3D bone organoids under mechanical load and it’s optimization using in-silico technologies. Further, the necessity of including immune cells into models of osteochondral tissues will be discussed. Examples about applications of dynamic magnetic fields to improve transfection and apply mechanical force inside living cells, a case study about a tissue-on-a-chip system for skin and intestine for applications in toxicology and aspects to employ living cells as biosensors in microphysiometrical systems are on the agenda.
At IEEE EMBC 2019 in Berlin / Germany cellasys co-organizes a symposia on:
Cells and tissue as prediction model for toxicology, drug development and personalized medicine ( organized by J. Wiest and F. Schulze )
M. Brischwein: Development of personalized therapies for cancer with 3D tumor spheroids
Multicellular tumor spheroids are 3D models generated from various cell types, including heterogeneous tissues of solid human tumors. They have proven utility for predictive testing of patient samples to identify the most promising pharmaceutical anti-cancer treatment.
F. Schulze: Mechanical loading of human osteoblasts in a bone-on-a-chip
Organ-on-chip systems are used to recreate the physiology of a certain tissue or organ of interest, thereby enabling more detailed investigations than conventional cell culture allows for. In this project a bone-on-a-chip system is presented that allows for control of oxygen levels and mechanical loading
A. Lang: Modelling the crosstalk between immune cells and bone
Interaction between immune cells and bone metabolism plays a crucial role in the pathogenesis of several disorders affecting the musculoskeletal system. However, current engineering approaches exclude the immune system due to its complexity. Here, we aim to present the importance of modelling the crosstalk between immune cells and bone as exemplified by mimicking the initial phase of fracture healing in vitro.
M. Koch: New magnetic field device for application with laser microscopes
A new digitally controlled field generator is integrated into a laser microscope. Magnetic particles powered non-invasively by the field generator treat cell populations inside a dish above the microscope lens, which can be observed in real time.
J. Wiest: Tissue-on-a-chip
Microphysiometry is a powerful tool to monitor the energy metabolism and changes in morphology of living cells. Until now, the technique was mainly used for monitoring of cells in 2D monolayers. Now, the cellasys group showed that it is possible to monitor the extracellular acidification rate (EAR) and transepithelial electrical resistance (TEER) of 3D skin constructs in an automated assay maintaining an air liquid interface (ALI) with the IMOLA-IVD technology.
The 2018 Annual Meeting of the Chinese Society of Biochemistry and Molecular Biology will take place from 25 – 28 October 2018 in Chongqing / China. Please visit our distribution partner Quantum Desing China in the exhibition.
Meet us at The Lush Prize Conference 2018.
Welcome to Berlin for The Lush Prize Conference 2018: Is there an end in sight for animal testing? Can Organ-on-a-Chip replace animal use in safety testing with advanced human focused approaches?
The 7th International Yeast 2.0 and Synthetic Genomes Conference will take place from 26 – 28 November 2018 in Sydney / Australia. Please visit our distribution partner Cell Systems Biology in the exhibition.
The meeting gathers together researchers, manufacturers, suppliers and others with an active interest in bioprocessing as applied to a broad range of industrial and academic research areas. Informative presentations and the opportunity to network and discuss new data, concepts and emerging trends in an informal environment is a feature of the conference.
Welcome our talk “Skin-on-a-chip with an automated air-liquid interface” and our poster “EpiIntestinal on a BioChip” at the 20th International Congress on In Vitro Toxicology (ESTIV 2018) in Berlin.
Skin-on-a-chip with an automated air-liquid interface
Wiest J., Eggert S., Alexander F.A.
We present a label-free solution that leverages the use of the intelligent mobile lab for in vitro diagnostics (IMOLA-IVD), a noninvasive, sensor-based platform, to monitor the transepithelial electrical resistance (TEER) of RhE models and adherent cells cultured on porous membrane inserts. Murine fibroblasts cultured on polycarbonate membranes were first used as a test model to optimize procedures using a custom BioChip encapsulation design, as well as dual fluidic configurations, for continuous and automated perfusion of membrane-bound cultures. Extracellular acidification rate (EAR) and TEER of membrane-bound L929 cells were monitored. The developed protocol was then used to monitor the TEER of MatTek EpiDermTM RhE models over a period of 48 h .
1 Alexander, F., Eggert, S., Wiest, J.: Skin-on-a-chip: Transepithelial electrical resistance and extracellular acidification measurements through an automated air-liquid interface, Genes, 9/2, 114; doi:10.3390/genes9020114 (2018).
EpiIntestinal on a BioChip
Wiest, J., Schmidt, Ch., Markus, J., Kandarova, H.
Microphysiometry showed to be a useful tool to monitor the energy metabolism of living cells and its interaction with living cells. In the past the technique was mainly used for monitoring of 2D monolayers of living cells . Recently, our group showed that it is also possible to monitor 3D hepatocyte spheroids  as well as the extracellular acidification rate (EAR) and transepithelial electrical resistance (TEER) of 3D skin constructs in an automated assay maintaining an air liquid interface (ALI) with the IMOLA-IVD technology . In this work we present an Intestine-on-a-BioChip by monitoring EAR and TEER of the MatTek 3D-small intestinal tissue model (EpiIntestinal) for 12 h. A periodic cycle of 96 min ALI, 10 min TEER measurement and 15 min washing step was used. The test substance (Triton-X) was applied after 8 h of measurement. After application of the tests substance a reduction of the EAR and the change in TEER could be monitored. To be able to monitor the EAR a low buffered basal medium was used. The presented work shows a proof of principle of automated monitoring of EAR and TEER at a 3D intestine model maintaining an automated ALI. The EpiIntestinal model on the IMOLA-IVD chip is a promising research tool for the use in the field of toxicology, cellular metabolism studies or drug absorption research.
1 Brischwein M., Wiest J. In: Bioanalytical Reviews, Springer, doi:10.1007/11663-2018-2 (2018).
2 Alexander, F., Eggert, S., Wiest, J.: A novel lab-on-a-chip platform for spheroid metabolism monitoring, Cytotechnology, 70/1, 375-386, doi:10.1007/s10616-017-0152-x (2018).
3 Alexander, F., Eggert, S., Wiest, J.: Skin-on-a-chip: Transepithelial electrical resistance and extracellular acidification measurements through an automated air-liquid interface, Genes, 9/2, 114; doi:10.3390/genes9020114 (2018).
Please visit our poster “EpiIntestinal on a Chip: Label-free Microphysiometry of Intestinal Epithelium” at the 21st European congress on alternatives to animal testing.
Please find a small series of reports about cellasys here:
cellasys is delighted to be shortlisted for the Lush Prize 2018 in the category science:
87758 Kronburg / Germany