Skin-on-a-Chip and EpiIntestinal-on-a-BioChip at ESTIV 2018

Welcome our talk “Skin-on-a-chip with an automated air-liquid interface” and our poster “EpiIntestinal on a BioChip” at the 20th International Congress on In Vitro Toxicology (ESTIV 2018) in Berlin.

 

Skin-on-a-chip with an automated air-liquid interface
Wiest J., Eggert S., Alexander F.A.

We present a label-free solution that leverages the use of the intelligent mobile lab for in vitro diagnostics (IMOLA-IVD), a noninvasive, sensor-based platform, to monitor the transepithelial electrical resistance (TEER) of RhE models and adherent cells cultured on porous membrane inserts. Murine fibroblasts cultured on polycarbonate membranes were first used as a test model to optimize procedures using a custom BioChip encapsulation design, as well as dual fluidic configurations, for continuous and automated perfusion of membrane-bound cultures. Extracellular acidification rate (EAR) and TEER of membrane-bound L929 cells were monitored. The developed protocol was then used to monitor the TEER of MatTek EpiDermTM RhE models over a period of 48 h [1].

1 Alexander, F., Eggert, S., Wiest, J.: Skin-on-a-chip: Transepithelial electrical resistance and extracellular acidification measurements through an automated air-liquid interface, Genes, 9/2, 114; doi:10.3390/genes9020114 (2018).

 

EpiIntestinal on a BioChip

Wiest, J., Schmidt, Ch., Markus, J., Kandarova, H.

Microphysiometry showed to be a useful tool to monitor the energy metabolism of living cells and its interaction with living cells. In the past the technique was mainly used for monitoring of 2D monolayers of living cells [1]. Recently, our group showed that it is also possible to monitor 3D hepatocyte spheroids [2] as well as the extracellular acidification rate (EAR) and transepithelial electrical resistance (TEER) of 3D skin constructs in an automated assay maintaining an air liquid interface (ALI) with the IMOLA-IVD technology [3]. In this work we present an Intestine-on-a-BioChip by monitoring EAR and TEER of the MatTek 3D-small intestinal tissue model (EpiIntestinal) for 12 h. A periodic cycle of 96 min ALI, 10 min TEER measurement and 15 min washing step was used. The test substance (Triton-X) was applied after 8 h of measurement. After application of the tests substance a reduction of the EAR and the change in TEER could be monitored. To be able to monitor the EAR a low buffered basal medium was used. The presented work shows a proof of principle of automated monitoring of EAR and TEER at a 3D intestine model maintaining an automated ALI. The EpiIntestinal model on the IMOLA-IVD chip is a promising research tool for the use in the field of toxicology, cellular metabolism studies or drug absorption research.

1 Brischwein M., Wiest J. In: Bioanalytical Reviews, Springer, doi:10.1007/11663-2018-2 (2018).

2 Alexander, F., Eggert, S., Wiest, J.: A novel lab-on-a-chip platform for spheroid metabolism monitoring, Cytotechnology, 70/1, 375-386, doi:10.1007/s10616-017-0152-x (2018).

3 Alexander, F., Eggert, S., Wiest, J.: Skin-on-a-chip: Transepithelial electrical resistance and extracellular acidification measurements through an automated air-liquid interface, Genes, 9/2, 114; doi:10.3390/genes9020114 (2018).

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